Design, synthesis and biological evaluation of indoline derivatives as multifunctional agents for the treatment of ischemic stroke

In this work, a series of indoline derivatives as multifunctional neuroprotective agents for battling ischemic stroke were designed, synthesized, and biologically evaluated. antioxidant assay, all compounds showed significant protective effects against H2O2-induced death RAW 264.7 cells. oxygen glucose deprivation/reperfusion (OGD/R)-induced neuronal damage, some significantly elevated the cell survival rate. Among them, 7i, 7j 7r exerted comparable to ifenprodil, exhibited binding affinity N-methyl-D-aspartic acid receptors 2B (NMDA-GluN2B). At concentrations 0.1, 1 10 μM, dose-dependently lowered LPS-induced secretion inflammatory cytokines, including TNF-α, IL-6 NO, by BV-2 Importantly, 7i can dramatically reduce cerebral infarction rate improve neurological deficit scores in middle artery occlusion (MCAO) rat model. As demonstrated above results, are potential treatment stroke.